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Trends are analysed by the Mann-Kendall method¹ in the free software R², using the wql package. This is a non-parametric test suggested by Mann (1945)³ and has been extensively used for environmental time series⁴. Mann-Kendall is a test for a monotonic trend in a time series y(x), which in this analysis is nutrient concentration (y) as a function of year (x). The size of the change is estimated by calculating the Sen slope^5,6. The same time series as for the time series analysis, but without gap filling.