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For building up the basis for an assessment, the list of substances was analysed in respect of different parameters. For this, each pesticide was checked in the Pesticides Properties Database (PPDP) (Lewis, et al., 2016). The outcome was a list with 180 pesticides including columns for chemical identifiers, their usage, the information about being it a parent substance or transformation product and their mode of action (MoA) to the pest organism (see Annex 5 and Annex 6). Based on this list, different assessments were made which are further shown in the paragraphs below and which are the basis for the development of a pesticide indicator to ease the river basin specific or site-specific monitoring and regulation of hot spots of contamination by a mixture of pesticides.